Catalog Number - 70200-05D-2

High‑Efficiency Bio‑Inert Diamond Hydride™ Performance for Fast LC‑MS

The 70200‑05D‑2 Bio‑Inert Diamond Hydride™ HPLC Column is a custom‑manufactured, high‑efficiency analytical column designed for laboratories requiring maximum sensitivity, fast separations, and metal‑free flow paths. Packed with 2.2 µm Diamond Hydride™ (Cogent TYPE‑C™ silica hydride) in bio‑inert, metal‑free stainless‑steel hardware and frits, this column delivers rapid equilibration, stable retention, and outstanding performance for highly polar and biologically sensitive compounds.

The 2.1 mm ID × 50 mm length format is optimized for short run times and LC‑MS workflows, where precise low‑flow control and minimized system volume are essential.

Why This Short, Narrow‑Bore Design Is Selected

This configuration is intentionally chosen for laboratories prioritizing speed and sensitivity:

• Short analysis times with minimal compromise in chromatographic efficiency

• Increased mass sensitivity due to narrow‑bore geometry

• Lower solvent consumption per run

• Bio‑inert hardware minimizes analyte–metal interactions that can degrade data quality

While compact in length, the 2.2 µm particle size provides high column efficiency, supporting sharp peaks and reliable retention for demanding analyses.

Diamond Hydride™ Stationary Phase Advantage

Built on Cogent TYPE‑C™ silica hydride technology, the Diamond Hydride™ stationary phase provides key advantages relative to traditional bonded‑silica HILIC materials:

• Very rapid equilibration, supporting high‑throughput workflows

• Stable and reproducible retention at elevated organic content

• Mixed‑mode interaction capability for difficult polar and zwitterionic analytes

• Long usable column lifetime, even under aggressive HILIC conditions

These characteristics make Diamond Hydride™ a preferred choice for routine and regulated LC‑MS methods.

Ideal Applications

The 70200‑05D‑2 bio‑inert Diamond Hydride column is routinely used for:

• Small, highly polar molecules

• Amino acids and organic acids

• Polar peptides and metabolites

• Proteins and other metal‑sensitive biological analytes

The metal‑free internal surfaces are particularly valuable when trace‑level accuracy and reproducibility are critical.

Fast LC‑MS and UV Detection

The 50 mm column length enables very fast separations and is especially effective for:

• LC‑MS methods requiring high sample throughput

• Screening and rapid method development workflows

• Applications where peak capacity can be traded for speed and sensitivity

For UV detection, system configuration must be optimized for low internal volume to maintain peak integrity.

LC‑MS Compatibility and System Considerations

This column format is a frequent LC‑MS favorite, particularly for HILIC‑based separations. Because of the 2.1 mm internal diameter and sub‑3 µm particle size, the system must support:

• Accurate and repeatable low‑flow delivery

• Minimized dead volume throughout the flow path

Standard HPLC systems lacking stable low‑flow capability may not be suitable.

Operating Guidelines and System Requirements

• Typical flow‑rate range: ~0.2–0.3 mL/min (method‑ and solvent‑dependent)

• System compatibility: HPLC or UHPLC systems capable of precise low‑flow control

• Special requirements: Low‑volume flow cell and optimized plumbing recommended

Flow rates may be tuned based on resolution, pressure limits, and detector configuration.

Custom Item Notice

This product is classified as a Custom Item. As with all Diamond Hydride™ prep, semi‑prep, and bio‑inert specialty columns, this item can be made to order and is non‑returnable.

• Learn more about Custom Orders and our Custom Order policies.